Before first use, thaw Re-Borne by placing it overnight in a refrigerator. When you're ready to use Re-Borne, just shake the bottle gently. No need to add water or mix. Shake the bottle each time you use Re-Borne. Begin by giving your horse 10 ml for 10 days. After 10 days, switch to five ml daily. You get the full benefit of Re-Borne through regular, daily use. After thawing and using Re-Borne, store it in the bottle in the refrigerator at 45oF (7oC). It can last for twelve months.